Synthesis of bilayer films from regenerated cellulose nanofibers and poly(globalide) for skin tissue engineering applications

Commercial cell-based skin regenerative products are highly expensive, carry the risk of rejection and require a long cell culture period to manufacture. This work describes synthesis bilayer films from poly(globalide) (PGl) regenerated cellulose nanofibers (rCNFs) their use as cell-free scaffold support keratinocyte attachment proliferation. The method is simple, eco-friendly (as precursor obtained agricultural waste) low cost. rCNFs were produced by acid hydrolysis PGl was via enzymatic ring-opening polymerization. synthesized layer-by-layer casting at ambient temperature. All showed well-defined interface between cellulose. rCNF/PGl metabolic activity far superior in comparison with pristine regarding growth, which illustrates potential these materials tissue engineering.